The use of Multiple Displacement Amplified DNA as a control for Methylation Specific PCR, Pyrosequencing, Bisulfite Sequencing and Methylation-Sensitive Restriction Enzyme PCR
نویسندگان
چکیده
منابع مشابه
Direct Bisulfite Sequencing and Methylation Specific PCR to Detect Methylation of p15INK4b and F7 genes in Coronary Artery Disease Patients
Genome-Wide Association Studies (GWAS) have identified genetic variants contributing to the risk of cardiovascular disease (CVD) at the chromosome 9p21 locus. The chromosome 9p21 is an important susceptibility locus for several multifactorial diseases like ischemic stroke, aortic aneurysm, type 2 diabetes mellitus and coronary artery disease (CAD). F7 gene because of its role in activating the ...
متن کاملdirect bisulfite sequencing and methylation specific pcr to detect methylation of p15ink4b and f7 genes in coronary artery disease patients
genome-wide association studies (gwas) have identified genetic variants contributing to the risk of cardiovascular disease (cvd) at the chromosome 9p21 locus. the chromosome 9p21 is an important susceptibility locus for several multifactorial diseases like ischemic stroke, aortic aneurysm, type 2 diabetes mellitus and coronary artery disease (cad). f7 gene because of its role in activating the ...
متن کاملStandard DNA methylation analysis in mouse epidermis: bisulfite sequencing, methylation-specific PCR, and 5-methyl-cytosine (5mC) immunological detection.
In mammals, methylation of cytosine C-5 position is a major heritable epigenetic mark on the DNA molecule. Maintenance of proper DNA methylation patterns is a key process during embryo development and in the maintenance of adult tissue homeostasis. The use of experimental procedures based on the chemical modification of cytosine by sodium bisulfite and the development of antibodies recognizing ...
متن کاملTwo Steps Methylation Specific PCR for Assessment of APC Promoter Methylation in Gastric Adenocarcinoma
Gastric Cancer (GC) is the second most common cancer in the world and a leading cause of cancer-related mortality. Methylation of promoter CpG islands (CGIs) belonging to tumor suppressor genes causes transcriptional silencing of their corresponding genes leading to carcinogenesis and other disorders. Adenomatous Polyposis Coli (APC) a tumor suppressor gene is inactivated by methylation of prom...
متن کاملControl of carry-over contamination for PCR-based DNA methylation quantification using bisulfite treated DNA
In this study, we adapted the well known uracil DNA glycosylase (UNG) carry-over prevention system for PCR, and applied it to the analysis of DNA methylation based on sodium bisulfite conversion. As sodium bisulfite treatment converts unmethylated cytosine bases into uracil residues, bisulfite treated DNA is sensitive to UNG treatment. Therefore, UNG cannot be used for carry-over prevention of ...
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ژورنال
عنوان ژورنال: BMC Molecular Biology
سال: 2007
ISSN: 1471-2199
DOI: 10.1186/1471-2199-8-91